Dl-Dithiothreitol ≥98% HPLC ≥99% Titration 25 Gram
Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
- CAS Number 3483-12-3
- Linear Formula HSCH2CH(OH)CH(OH)CH2SH
- Molecular Weight 154.25
- Beilstein/REAXYS Number 1719757
- EC Number 222-468-7
- MDL number MFCD00004877
- PubChem Substance ID 24894330
- NACRES NA.31
| grade | for molecular biology |
| assay | ≥98% (HPLC) |
| ≥99% (titration) | |
| mp | 41-44 °C (lit.) |
| solubility | H2O: 50 mg/mL |
| cation traces | heavy metals (as Pb): ≤5 ppm |
| foreign activity | DNase, RNase, protease, none detected |
| storage temp. | 2-8°C |
| SMILES string | O[C@H](CS)[C@H](O)CS |
| InChI | 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1 |
| InChI key | VHJLVAABSRFDPM-QWWZWVQMSA-N |
Application
DL-Dithiothreitol has been added to the samples before loading them for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used in the procedure of in situ hybridization of mRNA.
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
GHS07
Signal word Warning Hazard statements H302-H412 Precautionary statements P264-P270-P273-P301 + P312 + P330-P501 Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves RIDADR UN 3335 WGK Germany 3 RTECS EK1610000 Flash Point(F) 235.4 °F Flash Point(C) 113 °C
Documents
Safety Data Sheet
