Dulbecco’s Modified Eagle’s Medium – High Glucose, 500mL, case/6

Dulbecco’s Modified Eagle’s Medium – High Glucose, 500mL, Case/6

Synonym: DME, DMEM

• NACRES NA.75

Quality Level	300
sterility	sterile-filtered
form	liquid
application(s)	cell culture | hybridoma: suitable
	cell culture | mammalian: suitable
impurities	endotoxin, tested
components	glucose: highNaHCO3: yessodium pyruvate: noL-glutamine: nophenol red: yesHEPES: no
shipped in	ambient
storage temp.	2-8°C

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name DMEM/High.

Safety Information

NONH for all modes of transport

WGK Germany 3

Documents 

Safety Data Sheet