Trizma Base>= 99.0% (T), 500 Gram
Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
- CAS Number 77-86-1
- Linear Formula NH2C(CH2OH)3
- Molecular Weight 121.14
- Beilstein/REAXYS Number 741883
- EC Number 201-064-4
- MDL number MFCD00004679
- PubChem Substance ID 329770229
- NACRES NA.25
| description | aminopeptidase substrate |
| product line | BioChemika |
| assay | ≥99.0% (T) |
| form | crystalline |
| loss | ≤1% loss on drying, 110 °C |
| pH | 10.5-12.0(4 m in water, 25 °C) |
| useful pH range | 7 – 9 |
| pKa (25 °C) | 8.1 |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| 168-172 °C | |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| anion traces | chloride (Cl–): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| cation traces | Ca: ≤10 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| SMILES string | NC(CO)(CO)CO |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
General descriptionTris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
ApplicationTrizma base was used as buffer for the following studies:• Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase.• Electrophoretic separation of lipoproteins in polyacrylamide gels.• Preparation of TRIS buffer having pH 8.It may be used to compose DN buffer for DNA nick-end labeling of tissue sections.
Safety Information
Personal Protective Equipment dust mask type N95 (US), Eyeshields, GlovesRIDADR NONH for all modes of transportWGK Germany 1RTECS TY2900000
Documents
Safety Data Sheet
Warnings
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.
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